Generator
Part:BBa_K358010:Design
Designed by: Ken Kajita Group: iGEM10_Kyoto (2010-09-19)
anti-killer gene with GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 962
Design Notes
We performed three steps of PCR to prepare this part.
First, we got S gene from lambda phage DNA: λ -Hind Ⅲ digest, and inserted into the plasmid, BBa_E0840.
Then, performed the point mutation PCR, the same to the point mutation PCR on SRRz.
Finally, we deleted a transmembrane domain, TMD1, and constructed the anti-killergene: SΔTMD1.
Source
λ -Hind Ⅲ digest (product of TaKaRa Bio) and BBa_E0240
References
R. White, T. A. T. Tran, C. A. Dankenbring, J. Deaton, and R. Young, “The N-terminal transmembrane domain of lambda S is required for holin but not antiholin function,” Journal of Bacteriology, vol. 192, no. 3, pp. 725-733, Feb. 2010.